Large scale propagation of an exotic edible bamboo, Phyllostachys pubescens Mazel ex H. De Lehale (Moso Bamboo) using seeds.
نویسندگان
چکیده
For ex vitro propagation, seeds of P.pubescens were treated with different concentrations of gibberellic acid (GA3) and germination of seeds was tested both in plastic pots as well as by direct sowing in the nursery beds. Maximum seed germination was achieved when treated with 200 mgL(-1) (w/v) GA3. For in vitro propagation, an exposure of nodal explants from in vitro raised seedlings to 0.2 mgL(-1) 1-phenyl-3-(1,2,3-thiadiazol-5-yl) urea and 1 mgL(-1) kinetin supplemented medium for 30 days and thereafter to hormone free Murashige and Skoog basal medium resulted in axillary shoot proliferation. For rooting, in vitro raised shoots were exposed to MS medium containing 2 mgL(-1) indole-3-butyric acid for 15 days and then shifted to hormone free medium. On an average, 2.8 shoots were obtained in 75% of the cultures within 4 weeks. Such in vitro raised plants were successfully hardened and shifted to field conditions.
منابع مشابه
SELECTED PHYSICAL AND MECHANICAL PROPERTIES OF MOSO BAMBOO (Phyllostachys Pubescens)
YU, H. Q., JIANG, Z. H., HSE, C. Y. & SHUPE, T. F. 2008. Selected physical and mechanical properties of moso bamboo (Phyllostachys pubescens). Selected physical and mechanical properties of 4–6 year old moso bamboo (Phyllostachys pubescens) grown in Zhejiang, China were investigated at different vertical and horizontal positions. Two way analysis of variance and Tukey’s mean comparison tests in...
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Bamboo occupies an important phylogenetic node in the grass family and plays a significant role in the forest industry. We produced 1.2 Mb of tetraploid moso bamboo (Phyllostachys pubescens E. Mazel ex H. de Leh.) sequences from 13 bacterial artificial chromosome (BAC) clones, and these are the largest genomic sequences available so far from the subfamily Bambusoideae. The content of repetitive...
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متن کامل
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ورودعنوان ژورنال:
- Indian journal of experimental biology
دوره 52 7 شماره
صفحات -
تاریخ انتشار 2014